Cerebral vessels, neurovascular coupling and cerebral blood flow are the key targets in biomedical research and clinical diagnosis. We proposed high resolution laser speckle imaging techniques that can be used for in-vivo monitoring the neurovascular changes in real time. By on-line imaging processing, and miniature design of optics, we are able to extract the structural and functional features of the cerebral blood vessels. Based on the these techniques, we developed the imagers for different biomedical applications. Some examples will be illustrated:

1. intraoperative blood vessel imaging with real-time visualization, segmentation of cerebral blood vessels 2. imaging the blood flow and blood vessels changes in freely moving and conscious animals 3. studying the stroke formation  in conscious and freely moving state.